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- Title
In Vitro Activation of Human T Lymphocytes by <em>Haemophilus influenzae</em> Type b Capsular Polysaccharides.
- Authors
Peeters, C. C. A. M.; Tenbergen-Meekes, A. -M.; Heijnen, C. J.; Poolman, J. T.; Zegers, B. J. M.; Rijkers, G. T.
- Abstract
Polyribosilribitolphosphate (PRP), the capsular polysaccharide from Haemophilus influenzae type b. is a T-cell-independent type 2 antigen. In vitro culture of adult peripheral blood T cells with 15 μg/ml PRP leads to induction of inlerleukin-2 receptor (IL-2R) expression on up to 10%, of T cells. These cells are CD4+ and carry the αβ T-cell receptor. PRP, at concentrations above 1-5 μg/ml can also induce in vitro proliferation of both adult and neonatal T-cells. We conclude that PRP acts as a human T-cell mitogen. The in vitro proliferative response as well as IL-2R expression was studied in T-cells derived from adults after vaccination with native PRP, with PRP conjugated to a carrier protein, or with diphtheria toxoid. Vaccination with conjugated PRP decreased the doses of PRP required for in vitro induction of IL-2 R expression and T-cell proliferation. This indicates that vaccination with PRP conjugated to a carrier protein improves the in vitro T-cell response to PRP activation.
- Subjects
T cells; IMMUNIZATION; LEUCOCYTES; BIOPOLYMERS; LYMPHOCYTES; PROTEINS; CARRIER proteins
- Publication
Scandinavian Journal of Immunology, 1992, Vol 35, Issue 2, p137
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1992.tb02844.x