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- Title
Mechanisms in Neutrophil Priming: Characterization of the Oxidative Response Induced by Formylmethionyl-Leucyl- Phenylalanine in Human Exudated Cells.
- Authors
Follin, P.; Briheim, G.; Dahlgren, C.
- Abstract
Exudate human polymorphonuclear neutrophils were isolated and investigated regarding oxidative responsiveness and priming ability. The exudate neutrophils were found to produce an increased amount of O2- and H2O2 when stimulated with formylmethionyl-leucyl-phenylalanine (fMLP), i.e. these cells were metabolically primed. Cytochalasin B (cyt B) pretreatment affected the production of O2- by exudate cells, although to a lesser extent than the production by peripheral blood cells, in which a substantial increase was induced. Addition of N-ethylmaleimide (NEM) to activated exudate and peripheral blood cells revealed no difference in oxidase in activation rate. To induce further priming, the cells were incubated in vitro with a synthetic diacylglycerol (sn-1,2-didecanoylglycerol: diC10), or the Ca2+ ionophore ionomycin. Results of this procedure showed significant differences between exudate and peripheral blood neutrophils: the peripheral cells expressed a primed response, which was measured as increased fMLP-induced O2- production following incubation with both these substance: whereas the metabolic activity of exudated cells was not affected by diC10, but was significantly primed by ionomycin (P < 0.01), The exact route for diacylglycerol priming is unknown. However, our results with human neutrophils primed during exudation indicate an exhausted diC10-priming pathway, with a retained sensitivity for priming [Ca2+]I rises.
- Subjects
CELLS; BLOOD; NEUTROPHILS; GRANULOCYTES; AMINO acids; BLOOD cells; ORGANIC compounds
- Publication
Scandinavian Journal of Immunology, 1991, Vol 34, Issue 3, p317
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb01552.x