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- Title
The IgG FcRII and the PI-linked IgG FcRIII Trigger Cytoplasmic Calcium Fluxes Independently in Human Granulocytes.
- Authors
Lund-Johansen, F.; Olweus, Johanna; Aarli, Å.; Bjerknes, R.
- Abstract
The aim of this study was to investigate signal transduction through the two Fe receptors for IgG on human granulocytes. Using flow cytometry and the calcium indicator Fluo-3, we measured changes in leucocyte cytoplasmic calcium concentrations following cross-linking of cellular Fe receptors with specific antibodies. Two different approaches were used in order to study the two Fe receptors independently of each other. One was to avoid the presence of IgG Fe fragments, capable of binding to both types of receptors. The other was to use leucocytes from a patient with paroxysmal nocturnal haemoglobinuria (PNH) deficient in granulocyte FcRIII. In contrast to earlier reports, both approaches showed that the two types of IgG Fe receptors on granulocytes are capable of increasing cytoplasmic free calcium concentrations independently of each other. The results suggest that free cytoplasmic calcium ions are involved in the signal transduction pathway of both types of IgG Fe receptors on human granulocytes.
- Subjects
LEUCOCYTES; IMMUNOGLOBULIN G; CALCIUM; GRANULOCYTES; MICROBIAL genetics; KILLER cells; IMMUNOCOMPETENT cells
- Publication
Scandinavian Journal of Immunology, 1991, Vol 33, Issue 3, p261
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb01771.x