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- Title
Bone Regenerative Effect of Injectable Hypoxia Preconditioned Serum-Fibrin (HPS-F) in an Ex Vivo Bone Defect Model.
- Authors
Jiang, Jun; Röper, Lynn; Fuchs, Finja; Hanschen, Marc; Failer, Sandra; Alageel, Sarah; Cong, Xiaobin; Dornseifer, Ulf; Schilling, Arndt F.; Machens, Hans-Günther; Moog, Philipp
- Abstract
Biofunctionalized hydrogels are widely used in tissue engineering for bone repair. This study examines the bone regenerative effect of the blood-derived growth factor preparation of Hypoxia Preconditioned Serum (HPS) and its fibrin-hydrogel formulation (HPS-F) on drilled defects in embryonic day 19 chick femurs. Measurements of bone-related growth factors in HPS reveal significant elevations of Osteopontin, Osteoprotegerin, and soluble-RANKL compared with normal serum (NS) but no detection of BMP-2/7 or Osteocalcin. Growth factor releases from HPS-F are measurable for at least 7 days. Culturing drilled femurs organotypically on a liquid/gas interface with HPS media supplementation for 10 days demonstrates a 34.6% increase in bone volume and a 52.02% increase in bone mineral density (BMD) within the defect area, which are significantly higher than NS and a basal-media-control, as determined by microcomputed tomography. HPS-F-injected femur defects implanted on a chorioallantoic membrane (CAM) for 7 days exhibit an increase in bone mass of 123.5% and an increase in BMD of 215.2%, which are significantly higher than normal-serum-fibrin (NS-F) and no treatment. Histology reveals calcification, proteoglycan, and collagen fiber deposition in the defect area of HPS-F-treated femurs. Therefore, HPS-F may offer a promising and accessible therapeutic approach to accelerating bone regeneration by a single injection into the bone defect site.
- Subjects
FIBRIN; TERIPARATIDE; BONE density; BONE regeneration; HYPOXEMIA; CHORIOALLANTOIS; GROWTH factors; OSTEOPROTEGERIN
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 10, p5315
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25105315