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- Title
Alterations of monoamine neurotransmitters, HPA-axis hormones, and inflammation cytokines in reserpine-induced hyperalgesia and depression comorbidity rat model.
- Authors
Zhao, Jingjie; Shi, Wei; Lu, Yujia; Gao, Xuesong; Wang, Anna; Zhang, Shan; Du, Yi; Wang, Yongzhi; Li, Li
- Abstract
Background: Pain and depression often occur simultaneously, but the mechanism of this condition is still unclear. Methods: The aim of this study was to examine the alterations of monoamine neurotransmitters, hypothalamic–pituitary–adrenal (HPA) axis hormones, and inflammation cytokines in hyperalgesia and depression comorbidities. The reserpine-induced "Sprague Dawley" (SD) rat models were used, and the concentrations of monoamine neurotransmitters serotonin (5-HT), norepinephrine (NE), dopamine (DA), and their metabolic products 5-hydroxyindoleacetic acid (5-HIAA), Homovanillic acid (HVA), 3,4-Dihydroxyphenylacetic acid (DOPAC) in raphe nucleus region were tested by High Performance Liquid Chromatography (HPLC). Serum levels of Adrenocorticotropic Hormone (ACTH), Cortisol (CORT), and inflammatory cytokines interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, IL-4, IL-10 were assessed by enzyme linked immunosorbent assay. Results: Repeated reserpine injection induced hyperalgesia and depressive behaviors with decreased sucrose preference and horizontal movement distance, and increased immobility time in forced swimming test. The concentrations of 5-HT and NE in raphe nucleus, and ACTH and CORT in serum were elevated in the model group. And the model group showed increases in serum IL-1β and IL-6, and decrease in serum IL-10. Conclusion: More research in these areas is needed to understand the pathogenesis of the disease, so as to find more and better therapeutic targets.
- Subjects
TUMOR necrosis factors; NEUROTRANSMITTERS; HIGH performance liquid chromatography; ANIMAL disease models; CYTOKINES
- Publication
BMC Psychiatry, 2022, Vol 22, Issue 1, p1
- ISSN
1471-244X
- Publication type
Article
- DOI
10.1186/s12888-022-04065-0