We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Embryo development after mitochondrial supplementation from induced pluripotent stem cells.
- Authors
Li, Ruiqi; Wen, Bingqiang; Zhao, Haijing; Ouyang, Nengyong; Ou, Songbang; Wang, Wenjun; Han, Jianyong; Yang, Dongzi
- Abstract
Purpose: The purpose of this study was to evaluate the effects of mitochondrial supplementation (MS) on early embryonic development and to assess the safety of MS treatments using induced pluripotent stem cells (iPSCs) as the mitochondrial donor. Methods: In this study, we evaluated the effect of MS on early embryonic development using induced pluripotent stem cells (iPSCs) as the donor. Mouse zygotes were injected with either mitochondria from iPSCs or a vehicle solution. Several parameters were evaluated, including the rates of blastocyst formation and implantation, the weight of E13.5 embryos and placentas, the distribution of the donor mitochondrial DNA (mtDNA), and the pattern of methylation in the differentially methylated regions (DMRs) of the H19 and Snrpn genes. Results: We found that neither the rates of blastocyst formation and implantation nor the weights of E13.5 embryos and placentas were significantly different between the MS and control groups. Additionally, the mtDNA from the iPSC donors could be detected in the muscle tissue of four fetuses and all placentas in the MS group. Finally, the methylation patterns of H19 and Snrpn DMRs remained unchanged by MS. Conclusions: iPSC-derived mtDNA was directly involved in the process of embryonic development after MS. No adverse effects were seen when using iPSCs as a mitochondrial donor, but it remains to be seen whether this method can improve embryonic development, especially in older mice.
- Subjects
EMBRYOS; PLURIPOTENT stem cells; DNA methylation; MITOCHONDRIAL DNA; EMBRYOLOGY
- Publication
Journal of Assisted Reproduction & Genetics, 2017, Vol 34, Issue 8, p1027
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-017-0948-9