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- Title
Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana.
- Authors
Gao, Yongshun; Badejo, Adebanjo Ayobamidele; Shibata, Hitoshi; Sawa, Yoshihiro; Maruta, Takanori; Shigeoka, Shigeru; Page, Mike; Smirnoff, Nicholas; Ishikawa, Takahiro
- Abstract
The article presents a study which investigates the expression levels of both VTC2 and VTC5 gene encoding GDP-L-galactose phosphorylase, an enzyme involved in Ascorbate (AsA) Biosynthesis in Arabidopsis thaliana. The study used luciferase activity assay, RNA isolation, and bipyridyl method using microtitier plate reader. Results show that the transcript level of VTC2 and VTC5 have increased in parallel with AsA accumulation and VTC5 compensated on induced VTC2 transcription levels.
- Subjects
GENE expression; ARABIDOPSIS thaliana; GALACTOSE; PHOSPHORYLASES; LUCIFERASES; BIOSYNTHESIS
- Publication
Bioscience, Biotechnology & Biochemistry, 2011, Vol 75, Issue 9, p1783
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.110320