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- Title
LC-Orbitrap-HRMS method for analysis of traces of triacylglycerols featuring furan fatty acids.
- Authors
Wiedmaier-Czerny, Nina; Vetter, Walter
- Abstract
Furan fatty acids (FuFAs) are valuable antioxidants that are highly relevant for the protection of polyunsaturated fatty acids (PUFAs) in biological systems and food. Despite their low contributions to the total fatty acids, their widespread occurrence has been documented in food and biological samples. Like other fatty acids, FuFAs are also stored esterified, e.g., in triacylglycerols. However, FuFA-containing triacylglycerols had not been detected in lipidomics analyses. Here, we present a screening method that allows for the identification of traces of FuFA-containing triacylglycerols (TAGs) utilizing LC-Orbitrap-HRMS. Initially developed with the help of purposefully synthesized FuFA-containing TAGs, the screening method was successfully applied to the analysis of two fish oil samples and one mushroom extract sample. Several FuFA-containing TAGs could be identified by direct analysis using the method and database developed in this study.
- Subjects
FATTY acids; ANALYSIS of triglycerides; TRACE analysis; UNSATURATED fatty acids; FISH oils
- Publication
Analytical & Bioanalytical Chemistry, 2023, Vol 415, Issue 5, p875
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-022-04480-y