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- Title
Redirection of the Transcription Factor SP1 to AT Rich Binding Sites by a Synthetic Adaptor Molecule.
- Authors
Bolz, Mathias; Scheffer, Ute; Kalden, Elisabeth; Göbel, Michael W.
- Abstract
The ubiquitous transcription factor SP1 binds to a GC rich consensus sequence. Here we describe an adaptor molecule that mediates binding of SP1 to a non‐cognate DNA site rich in AT. The adaptor is comprised of a Dervan‐type hairpin polyamide with high affinity to an AT rich hexamer duplex. It also carries a 27mer DNA that contains the SP1 consensus sequence. The synthesis and purification of the polyamide‐DNA conjugate is reported. Pulldown experiments and western blot analysis demonstrate adaptor mediated binding of SP1 to the hexamer duplex TTGTTA.
- Subjects
TRANSCRIPTION factor Sp1; BINDING sites; TRANSCRIPTION factors; WESTERN immunoblotting; MYELOID differentiation factor 88; MOLECULES
- Publication
Helvetica Chimica Acta, 2021, Vol 104, Issue 9, p1
- ISSN
0018-019X
- Publication type
Article
- DOI
10.1002/hlca.202100095