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- Title
Indocyanine Green Fluorescence Measurement of Intestinal Transit and Gut Perfusion after Intestinal Manipulation.
- Authors
Behrendt, F. F.; Tolba, R. H.; Overhaus, M.; Hirner, A.; Minor, T.; Kalff, J. C.
- Abstract
Background and Aims: Postoperative ileus is a common and poorly understood problem of abdominal surgery. The aim of this study was to measure postoperative intestinal transit and to evaluate bowel wall perfusion by a novel in vivo indocyanine green (ICG)-fluorescence measurement following intestinal manipulation (IM). Methods: Rats underwent a simple intestinal manipulation. Myeloperoxidase-positive cells in the muscularis were stained with the Hanker-Yates reaction and quantified histochemically. Bowel wall perfusion was determined directly and 24 h postoperatively using a laser-fluorescence detection unit. Intestinal transit was visualized 24 h after IM. Results: IM resulted in a massive infiltration (155-fold) of neutrophils into the intestinal muscularis 24 h postoperatively. Bowel wall perfusion significantly decreased directly and 24 h following surgery (29 and 59%, respectively). Gastrointestinal transit was similarly impaired and showed a reduction to 40% of the control values 24 h after IM. Conclusion: IM of the rat small intestine caused an impairment in bowel wall perfusion and microcirculation and a significant decrease in gastrointestinal transit. The ICG fluorescence measurement using the described system proved to be a simple and reliable method to evaluate intestinal transit and bowel wall microcirculation in vivo. Copyright © 2004 S. Karger AG, Basel
- Subjects
BOWEL obstructions; INDOCYANINE green; ABDOMINAL surgery; HETEROCYCLIC compounds; FLUORESCENCE; PERFUSION
- Publication
European Surgical Research, 2004, Vol 36, Issue 4, p210
- ISSN
0014-312X
- Publication type
Article
- DOI
10.1159/000078855