We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Study on in vitro antibacterial and anti-inflammatory effects of fermented flavones from the stem and leaves of Scutellaria baicalensis.
- Authors
LIN Jun-yi; SONG Chun-lian; ZHANG Ying; ZHANG Zhi-hui; QUAN Wei; SHU Xiang-hua
- Abstract
The aim of the experiment was to investigate the antibacterial and anti-inflammatory effects of fermented flavones from the stem and leaf of Scutellaria baicalensis, Escherichia coli, Salmonella, Shigella, and Staphylococcus aureus were used as test strains, the MIC, MBC, and inhibitory circle size were determined. A total of 50 SPF grade Kunming mice were randomly divided into five groups with 10 mice in each group. Mice in all groups except the control group (Con group) were intraperitoneally injected with lipopolysaccharide (LPS, 4 mg/kg), and mice in the Con group were administrated with the same dose of normal saline. The positive drug group (LPS + DM group) was given 0.5 mg/kg dexamethasone. The original drug group (LPS + SBSL group) and fermentation drug group (LPS + FSBSL group) were fed the 8 mg/kg original drug and fermentation flavones. The experiment lasted for seven days. The results showed that the lowest MIC and MBC values of fermented flavones were Escherichia coli and Staphylococcus aureus, respectively. At 250 g/L, fermented flavones showed moderate sensitivity to Escherichia coli, Shigella, and Staphylococcus aureus. Compared with the LPS group, the mortality of mice in the LPS + FSBSL group was significantly reduced. The liver index in the LPS + SBSL group was extremely higher than that in the LPS + FSBSL group (P<0.01), and the spleen index in the LPS group was extremely higher than that in the Con group (P<0.01). Compared with the LPS group, the serum IL-6 content in the LPS + DM group and LPS + SBSL group was extremely increased (P<0.01). Compared with the LPS + DM group, the serum IL-6 content of mice in the LPS + SBSL group and LPS + FSBSL group was extremely increased (P<0.01). Compared with the Con group, the serum TNF-α content of mice in the LPS group was significantly increased (P<0.05). Compared with the LPS group, the content of IL-6 in the lung tissue of mice in the LPS + FSBSL group was significantly increased (P<0.05). Compared with the LPS + SBSL group, the content of IL-1β in the lung tissue of mice in the LPS + FSBSL group was significantly increased (P<0.05). The study indicates that fermented flavones from the stem and leaf of Scutellaria baicalensis have a good antibacterial effect on Shigella and Staphylococcus aureus, and the safe dose is 8 mg/kg, which significantly inhibite the mortality of mice infected with LPS, and affects the release of inflammatory factors IL-6, IL-1β, and TNF-α in the serum and lung of mice infected with LPS.
- Subjects
SCUTELLARIA; CHINESE skullcap; FLAVONES; STAPHYLOCOCCUS aureus; LIPOPOLYSACCHARIDES; IN vitro studies; ESCHERICHIA coli
- Publication
Feed Research, 2024, Vol 47, Issue 1, p61
- ISSN
1002-2813
- Publication type
Article
- DOI
10.13557/j.cnki.issn1002-2813.2024.01.012