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- Title
Structure characteristics and antioxidant activity of alfalfa polysaccharides prepared by ultrasonic assisted method.
- Authors
LI Fengwei; MU Na; LIU Xiaochen; WANG Dujun; SHANG Yueling; YU Xiaohong
- Abstract
The ultrasonic assisted method was used to prepare alfalfa crude polysaccharides. A homogeneous polysaccharides APSU-2a was purified by DEAE-52 cellulose and Sephadex G-75 gel column chromatography. Structure characterization of APSU-2a was analyzed by high performance gel permeation chromatography, precolumn PMP derivatization and high performance liquid chromatography, Fourier transform infrared spectrum (FTIR) and nuclear magnetic resonance spectroscopy (NMR) . Its in vitro antioxidant activity was evaluated by scavenging ability of DPPH, ABTS and OH radicals. The results showed that the molecular weight of APSU-2a was 311.7kDa and was mainly composed of four monosaccharides: mannose, rhamnose, galactose and arabinose, with a small amount of glucuronic acid and galacturonic acid. FTIR and NMR spectroscopy analysis showed that APSU- 2a exhibited typical absorption peaks of polysaccharides, it also contained α-type and β-type glycosidic bonds. APSU-2a had a good antioxidant activity, within a certain concentration range, its in vitro antioxidant activity is concentration dependent. APSU-2a can be used as a functional ingredient with antioxidant ability in the development of functional foods.
- Subjects
POLYSACCHARIDES; MONOSACCHARIDES; NUCLEAR magnetic resonance spectroscopy; HIGH performance liquid chromatography; GEL permeation chromatography; ALFALFA; GALACTURONIC acid
- Publication
China Food Additives, 2022, Vol 33, Issue 3, p157
- ISSN
1006-2513
- Publication type
Article
- DOI
10.19804/j.issn1006-2513.2022.03.021