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- Title
ŠTÚDIUM POSTUPOV PREDÚPRAVY MATERSKÉHO MLIEKA PRE HPLC-MS/MS STANOVENIE VYBRANÝCH METABOLITOV PLASTIFIKÁTORA DINCH.
- Authors
Helena, JURDÁKOVÁ; Alžbeta, ORAVCOVÁ; Renáta, GÓROVÁ
- Abstract
Background: Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) gradually replaces phthalates used as plasticizers in many consumer products due to its more favorable toxicological profile. However, as the DINCH production and usage have been rapidly increased, the rate of exposure is increased accordingly, so biomonitoring of exposure is important mainly in sensitive population cohorts such as children in early life. By breastfeeding, the mother can transfer potentially toxic chemicals to which the mother has previously been exposed. As biomarkers of exposure to DINCH mainly two oxidized metabolites, hydroxy-(OH-MINCH) and carboxy- (cx-MINCH) cyclohexane-1,2-dicarboxylic acid monoesters are used. Aim: The aim of this work was to study breast milk sample pretreatment prior to HPLC-MS/MS determination of these two metabolites. Methods: Pretreatment procedures were assessed for overall pretreatment efficiency based on the recovery of cx-MINCH and OH-MINCH from the matrix and on the matrix effects in the ionization source of mass spectrometer. The aqueous milk fraction obtained after centrifugation and extracts into ethyl acetate and ethyl acetate/hexane were analyzed. Results: Pretreatment efficiency obtained for aqueous milk fraction and ethylacetate extracts were very similar at the level of 50 %, after adding hexane into the extraction solution the recovery decreased by half. Conclusion: Extraction to examined organic solvents did not bring any improvement of pretreatment method efficiency, the procedure was more laborious and time consuming. On the basis of HPLC-MS/MS analysis of aqueous milk fraction we estimated limits of quantification for cx-MINCH 0.03 µg/l and for OH-MINCH 0.12 µg/l.
- Publication
Zdravotnicke listy, 2023, Vol 11, Issue 4, p63
- ISSN
1339-3022
- Publication type
Article