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- Title
A highly efficient method for generation of therapeutic quality human pluripotentstem cells by using naive induced pluripotent stemcells nucleus for nuclear transfer.
- Authors
Sanal, Madhusudana Girija
- Abstract
Even after several years since the discovery of human embryonic stem cells and induced pluripotent stem cells, we are still unable to make any significant therapeutic benefits out of them such as cell therapy or generation of organs for transplantation. Recent success in somatic cell nuclear transfer made it possible to generate diploid embryonic stem cells, which opens up the way to make high-quality pluripotent stem cells. However, the process is highly inefficient and hence expensive compared to the generation of induced pluripotent stem cells. Even with the latest somatic cell nuclear transfer technology, we are not sure whether one can make therapeutic quality pluripotent stem cell from any patient's somatic cells or by using oocytes from any donor. Combining induced pluripotent stem cells technology with somatic cell nuclear transfer, that is, by using the nucleus of the candidate somatic cell which got reprogrammed to pluripotent state instead that of the unmodified nucleus of the candidate somatic cell, would boost the efficiency of the technique, and we would be able to generate therapeutic quality pluripotent stem cells. Induced pluripotent stem cell nuclear transfer combines the efficiency of induced pluripotent stem cells (iPSC) generation with the speed and natural reprogramming environment of somatic cell nuclear transfer (SCNT). The new technique may be called induced pluripotent stem cell nuclear transfer (iPSCNT). This technique could prove to have very revolutionary benefits for humankind. This could be useful in generating organs for transplantation for patients and for reproductive cloning, especially for childless men and women who cannot have children by any other techniques. When combined with advanced gene editing techniques (such as CRISPR-Cas system) this technique might also prove useful to those who want to have healthy children but suffer from inherited diseases. The current code of ethics may be against reproductive cloning. However, this will change with time as it happened with most of the revolutionary scientific breakthroughs. After all, it is the right of every human to have healthy offspring and it is the question of reproductive freedom and existence.
- Publication
SAGE Open Medicine, 2014, Vol 2, p1
- ISSN
2050-3121
- Publication type
Article
- DOI
10.1177/2050312114550375