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- Title
The sequence and de novo assembly of Takifugu bimaculatus genome using PacBio and Hi-C technologies.
- Authors
Zhou, Zhixiong; Liu, Bo; Chen, Baohua; Shi, Yue; Pu, Fei; Bai, Huaqiang; Li, Leibin; Xu, Peng
- Abstract
Takifugu bimaculatus is a native teleost species of the southeast coast of China where it has been cultivated as an important edible fish in the last decade. Genetic breeding programs, which have been recently initiated for improving the aquaculture performance of T. bimaculatus, urgently require a high-quality reference genome to facilitate genome selection and related genetic studies. To address this need, we produced a chromosome-level reference genome of T. bimaculatus using the PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. The genome was assembled into 2,193 contigs with a total length of 404.21 Mb and a contig N50 length of 1.31 Mb. After chromosome-level scaffolding, 22 chromosomes with a total length of 371.68 Mb were constructed. Moreover, a total of 21,117 protein-coding genes and 3,471 ncRNAs were annotated in the reference genome. The highly accurate, chromosome-level reference genome of T. bimaculatus provides an essential genome resource for not only the genome-scale selective breeding of T. bimaculatus but also the exploration of the evolutionary basis of the speciation and local adaptation of the Takifugu genus. Measurement(s) whole genome sequencing assay • transcription profiling assay Technology Type(s) DNA sequencing • RNA sequencing Factor Type(s) organism part Sample Characteristic - Organism Takifugu bimaculatus Machine-accessible metadata file describing the reported data: 10.6084/m9.figshare.9793001
- Subjects
ANIMAL breeding; ANIMAL genetics techniques; AQUACULTURE; CHROMOSOMES; GENETIC transcription; RNA sequencing
- Publication
Scientific Data, 2019, Vol 6, Issue 1, pN.PAG
- ISSN
2052-4463
- Publication type
Article
- DOI
10.1038/s41597-019-0195-2