We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Proliferation of the Co-culture System Composed of Adipose-Derived Stromal Cells and Vascular Endothelial Cells Marked by BrdU on Bone Tissue Engineering Bone in Vivo.
- Authors
ZHAO Yu-xin; WANG Fu-ke; LI Yan-lin; ZHANG Hong; WANG Guo-liang; HE Chuan
- Abstract
Objective The purpose of this study was to investigate the proliferation and differentiation of the co-culture system composed of adipose-derived stem cells (ADSCs) and vascular endothelial cells (VECs) on bone tissue engineering bone in vivo. Methods (1) The PDPB materials were made with porcine spine bone. It was modified by fibronectin to make partially deproteinised biologic bone (PDPBB) , and then the surface information of PDPBB was observed with a scanning electron microscope. (2) Rat ADSCs and VECs induced differentiation from mononuclear cells from umbilical cord blood in rats were isolated and co-cultured by the proportion of 1:1 in vitro. (3) VECs, ADSCs and co-cultured cells marked by BrdU were compounded separately with the PDPBBs in vitro, and the tissue engineering bones were transplanted into both sides of femoral muscles bags in SD rats after cultured 6 days. The immunofluorescence staining of Brdu antibody was separately carried on in order to observe the proliferation and differentiation of the seeds implanted cells after 2 weeks, 4 weeks, 8 weeks and 12 weeks in vivo. Results (1) PDPB with pore distribution prepared by pig spinal was made by a large number of hydroxyapatite fibers woven. Partially deproteinized bone surface showed a large number of granular biological protein crystallization. (2) Using BrdU immunofluorescence staining, seed cells implanted in each group disseminated in the beginning, and then cell gradually proliferated in a nesting pattern. VECs and co-cultured cell groups formed a large number of new vessels after implanting endothelial cells. No positive cell was observed in the unmaked BrdU control group. Conclusion The seed cells implanted in the tissue engineered bone can be proliferated in vivo, and participate in the formation of new tissues and vessels.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2014, Vol 35, Issue 3, p13
- ISSN
2095-610X
- Publication type
Article