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- Title
Development and Validation of a Simple Method to Quantify Contents of Phospholipids in Krill Oil by Fourier-Transform Infrared Spectroscopy.
- Authors
Park, Se-Eun; Yu, Hyo-Yeon; Ahn, Sangdoo
- Abstract
This study focuses on developing a quantification method for phosphatidylcholine (PC) and total phospholipid (PL) in krill oil using Fourier-transform infrared (FT-IR) spectroscopy. Signals derived from the choline and phosphate groups were selected as indicator variables for determining PC and total PL content; calibration curves with a correlation coefficient of >0.988 were constructed with calibration samples prepared by mixing krill oil raw material and fish oil in different ratios. The limit of detection (LOD, 0.35–3.29%) of the method was suitable for the designed assay with good accuracy (97.90–100.33%). The relative standard deviations for repeatability (0.90–2.31%) were acceptable. Therefore, both the methods using absorbance and that using second-derivative were confirmed to be suitable for quantitative analysis. When applying this method to test samples, including supplements, the PC content and total PL content were in good agreement with an average difference of 2–3% compared to the 31P NMR method. These results confirmed that the FT-IR method can be used as a convenient and rapid alternative to the 31P NMR method for quantifying PLs in krill oil.
- Subjects
INFRARED spectroscopy; KRILL oil; PHOSPHOLIPIDS; LECITHIN; STANDARD deviations; FISH oils; EDIBLE fats &; oils; NEAR infrared spectroscopy
- Publication
Foods, 2022, Vol 11, Issue 1, p41
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods11010041