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- Title
山东地区樱桃褐斑病病原鉴定及rDNA-ITS序列分析.
- Authors
孙 杨; 韩 伟; 孙玉刚; 魏国琴; 付全娟; 杨兴华; 宫庆涛
- Abstract
In order to clarify the pathogenic species of cherry brown leaf spot, this study isolated and purified 16 fungal strains by single spore separation method. It also collected typical morbidity leaf blades from Taian, Weifang and Yantai 3 regions. Identification of cherry brown leaf spot pathogen was carried out by pathogen morphology combined with molecular biology method. The ITS sequence analysis of this pathogen showed that it was aligned with Genbank and showed 97% identity with Passalora arctostaphyli. The results of phylogenetic analysis showed that the pathogen was located at the same branch of phylogenetic tree with Passalora sp. and different from Pseudocercospora sp. According to its morphological characteristics and the sequence of internal transcribed spacer, the pathogen was identified as Passalora circumscissa, and ITS gene sequence was presented to GenBank database(Genbank Accession No.: KT428056) .
- Subjects
MOLECULAR biology; SEQUENCE analysis; DATABASES; TREE branches; SPORES; LEAF spots
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2017, Vol 19, Issue 7, p44
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2016.677