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- Title
Curcumin inhibits MCF-7 cells by modulating the NF-κB signaling pathway.
- Authors
JUN-LI LIU; YAN-YAN PAN; OU CHEN; YUN LUAN; XIA XUE; JING-JIE ZHAO; LING LIU; HONG-YING JIA
- Abstract
The present study investigated the inhibitory effect of curcumin on human breast cancer MCF-7 cells and investigated the potential underlying molecular mechanisms. MCF-7 cells were cultured with curcumin at different concentrations and time points. The effects of curcumin treatment on breast cancer cell proliferation were studied using a MTT assay. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to assess the mRNA and protein expression levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax), nuclear factor-κ-light-chain-enhancer of activated B cells (NF-κB) and inhibitor of NF-κB-α (IκBα). The proliferation of MCF-7 cells in the group treated with curcumin was markedly decreased compared with the control, with the greatest inhibitory effect at a concentration of 20 µM. The expression of Bax mRNA was increased and Bcl-2 mRNA expression was decreased compared with the control. Additionally, protein expression of NF-κB and IκB was increased. The data indicate that curcumin is able to inhibit breast cancer cell proliferation, possibly by regulating the NF-κB signaling pathway.
- Subjects
CURCUMIN; BREAST cancer treatment; NF-kappa B; CELLULAR signal transduction; CANCER cell culture; PROTEIN expression; THERAPEUTICS
- Publication
Oncology Letters, 2017, Vol 14, Issue 5, p5581
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2017.6860