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- Title
Optimization of the Green Synthesis of Silver Nanoparticle with Box-Behnken Design: Using Aloe Vera Plant Extract as a Reduction Agent.
- Authors
TOSUN, Nazan GÖKŞEN; KAPLAN, Özlem
- Abstract
Nowadays, many of plants are used as a reduction agent in biosynthesis of silver nanoparticles. In this study, green synthesis of silver nanoparticles (AgNPs) was aimed to optimize with Box- Behnken design (BBD). Aloe vera plant extract was utilized as a reduction agent as it is the famous natural product in field of cosmetic and skin health care. The synthesized AgNPs using Aloe vera plant extract solution was optimized by Box-Behnken design using the influence of different factors such as microwave power, time, AgNO3 concentration, and ratio of volume of Aloe vera plant extract solution to volume of AgNO3 and the percentage yield of particle formation as a response. Quadratic polynomial model was used to carry out mathematical modelling and response surface analysis was performed to determine the independent variable- response relationship. The optimized silver nanoparticles were characterized using FTIR spectroscopy and UV-VIS spectrophotometry. The antibacterial activity of optimized AgNPs was determined by testing against Gram positive (Staphylococcus aureus, Enterococcus faecalis) and Gram negative (Pseudomonas aeruginosa and Klebsiella pneumoniae) bacteria. Briefly, the trials interpreted successful synthesis of the AgNPs along with comprehension of the related factors affecting their quality characteristics and remarkably developed antibacterial activity as helpful impact.
- Subjects
SILVER nanoparticles; PLANT extracts; NANOPARTICLE synthesis; ALOE vera; FOURIER transform infrared spectroscopy; ENTEROCOCCUS faecalis; NATURAL products
- Publication
Sakarya University Journal of Science (SAUJS) / Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2021, Vol 25, Issue 3, p774
- ISSN
1301-4048
- Publication type
Article
- DOI
10.16984/saufenbilder.806916