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- Title
Protein and carbohydrate accumulation in normal and high-lysine barley in spike culture.
- Authors
Mather, Diane E.; Giese, Henriette
- Abstract
Spikes of barley (Hordeum vulgare L.) cultivar Bomi and high-lysine mutants Riso 1508 and Riso 56 were cultured on liquid media at varying N and sucrose levels. Bomi accumulated N in response to increasing N levels in the medium and a higher level was reached than in spikes of intact plants. The distribution of N in salt-soluble, hordein, and non-protein N fractions appeared to be normal. Endosperm dry weight and starch were lower than in intact plants and declined at higher N levels. A linear relationship was observed between starch content and the concentration of sucrose in the endosperm water. Uptake of culture medium by the spikes was affected by both N and sucrose concentration. The mutants had lower dry weights and starch contents, and higher sucrose contents than Bomi. At high N levels, the mutants accumulated less hordein, and more lion-protein N than Bomi.
- Subjects
BARLEY; CARBOHYDRATE metabolism; PROTEINS; SUCROSE; PLANT growing media; PLANT physiology
- Publication
Physiologia Plantarum, 1984, Vol 60, Issue 1, p75
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1984.tb04253.x