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- Title
Determination of novobiocin by a kinetic spectrophotometric method in milk and biological samples.
- Authors
Keyvanfard, Mohsen; Alizad, Khadijeh; Najjarian, Nazila
- Abstract
A new, simple, inexpensive, and fast kinetic spectrophotometric method was developed for the determination of trace amounts of antibiotic drug novobiocin sodium over the range of 4-32 ng/mL. The method is based on the inhibitory effect of novobiocinon the reaction of Amido Black and periodate in the acidic micellar mediums. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance at 627 nm and fixed-time 6 min from initiation of the reaction. The detection limit is 3 ng/mL, and relative standard deviation for 4.8 and 12 ng/mL novobiocin were 0.52 and 0.65%, respectively, for six replicate measurements. The method was applied to the determination of novobiocin in human and dog serum, urine, and milk samples.
- Subjects
NOVOBIOCIN; SPECTROPHOTOMETRY; MILK analysis; ANTIBIOTICS; BLOOD serum analysis
- Publication
Journal of Analytical Chemistry, 2017, Vol 72, Issue 10, p1028
- ISSN
1061-9348
- Publication type
Article
- DOI
10.1134/S1061934817100124