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- Title
The Energies of Activation and Deactivation of 2,4-Dichlorophenol Degradation by Horseradish Peroxidase Immobilized on the Modified Nanofibrous Membrane.
- Authors
Miłek, Justyna
- Abstract
Featured Application: The obtained results can be used in the design and modeling of industrial degradation of 2,4-dichlorophenol when using an enzyme membrane with horseradish peroxidase. Chlorophenol compounds pose a health risk to many organisms due to their toxicity. The present paper presents the estimation of the activation and deactivation energies and the optimum temperatures of 2,4-dichlorophenol degradation by horseradish peroxidase (HRP). The activities of horseradish peroxidase depending on temperature were analyzed. In a mathematical model, describing 2,4-dichlorophenol degradation by HRP was assumed that both the 2,4-dichlorophenol degradation and the deactivation of HRP were first-order reactions by the enzyme concentration. The parameters of the optimum temperatures T o p t , the activation energies E r , and the deactivation energies E d in the process of 2,4-dichlorophenol degradation by HRP immobilized on a modified nanofibrous membrane were determined k d and t 1 / 2 were determined for HRP immobilized at temperatures in the range of 25 °C to 75 °C. Likewise, thermodynamic parameters such as the change in the enthalpy ∆ H # , change in entropy ∆ S # , the change in Gibbs free energy ∆ G # for native HPR and the change in the enthalpy ∆ H d # , change in entropy ∆ S d # , and the change in Gibbs free energy ∆ G d # for deactivated HRP were determined at 25 °C.
- Subjects
HORSERADISH peroxidase; ACTIVATION energy; GIBBS' free energy
- Publication
Applied Sciences (2076-3417), 2024, Vol 14, Issue 6, p2423
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app14062423