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- Title
Retrograde Intraflagellar Transport Mutants Identify Complex A Proteins With Multiple Genetic Interactions in Chlamydomonas reinhardtii.
- Authors
lomini, Carlo; Linya Li; Esparza, Jessica M.; Dutcher, Susan K.
- Abstract
The intraflagellar transport machinery is required for the assembly of cilia. It has been investigated by biochemical, genetic, and computational methods that have identified at least 21 proteins that assemble into two subcomplexes. It has been hypothesized that complex A is required for retrograde transport. Temperature-sensitive mutations in FLA15 and FLA17 show defects in retrograde intraflagellar transport (IFT) in Chlamydomonas. We show that 1FT144 and 1FT139, two complex A proteins, are encoded by FL415 and FLA17, respectively. The fla15 allele is a missense mutation in a conserved cysleine and the fla17 allele is an in-frame deletion of three exons. The flagellar assembly defect of each mutant is rescued by the respective transgenes. In fla15 and fla17 mutants, bulges form in the distal one-third of the flagella at the permissive temperature and this phenotype is also rescued by the transgenes. These bulges contain the complex B component 1FT74/72, but not α-tubulin or p28, a component of an inner dynein arm, which suggests specificity with respect to the proteins that accumulate in these bulges. 1FT144 and 1FT139 are likely to interact with each other and other proteins on the basis of three distinct genetic tests: (1) Double mutants display synthetic flagellar assembly defects at the permissive temperature, (2) heterozygous diploid strains exhibit second-site noncomplemention, and (3) transgenes confer two-copy suppression. Since these tests show different levels of phenotypic sensitivity, we propose they illustrate different gradations of gene interaction between complex A proteins themselves and with a complex B protein (1FT172).
- Subjects
GENOTYPE-environment interaction; GENETIC mutation; CHLAMYDOMONAS reinhardtii; FLAGELLARIACEAE; PROTEINS; GENETIC research
- Publication
Genetics, 2009, Vol 183, Issue 3, p885
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.109.101915