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- Title
EXPOSURE OF RAW-264.7 MACROPHAGE CELL LINE TO WATER-SOLUBLE EXTRACT OF CHEDDAR CHEESE: ASSESSMENT OF ANTIOXIDANT ACTIVITY.
- Authors
Rafiq, S.; Huma, N.; Pasha, I.; Gulzar, N.; Shahid, M.; Xiao, H.
- Abstract
Oxidative stress is the most critical factor implicated in various degenerative ailments. The present study was designed to evaluate the in vitro radical scavenging potential of water-soluble peptides (WSPs) extract derived from buffalo and cow milk cheddar cheeses during ripening. Moreover, the protective role of WSPs extract on cell viability and production of reactive oxygen species (ROS) in RAW-264.7 macrophages (Lipopolysaccharide- stimulated) cell line was also assessed. The WSPs extracts were potent scavenger of DPPH radical and activity increased progressively with cheese ripening period. Comparatively, highest activity was noticed in the peptide extract obtained from buffalo milk cheddar cheese. The intracellular ROS production in RAW-264.7 macrophages decreased significantly (P<0.05) upon treatment with WSPs extract. However, the WSPs fraction collected at 150th day of ripening showed maximum reduction in ROS generation. Additionally, dose-dependent response of WSPs extract was noticed for antioxidant activity. The promising antioxidant potential of cheddar cheese can offer a perspective to reduce the risk of disorders associated with oxidative damage.
- Subjects
CHEDDAR cheese; MACROPHAGES; OXIDANT status; OXIDATIVE stress; REACTIVE oxygen species
- Publication
JAPS: Journal of Animal & Plant Sciences, 2017, Vol 27, Issue 2, p611
- ISSN
1018-7081
- Publication type
Article