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- Title
Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files.
- Authors
Heggendorn, Fabiano Luiz; Fraga, Aline Guerra Manssour; Ferreira, Dennis de Carvalho; Gonçalves, Lucio Souza; Lione, Viviane de Oliveira Freitas; Lutterbach, Márcia Teresa Soares
- Abstract
<italic>Aim</italic>. This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. <italic>Methods</italic>. Sulfate-reducing bacteria (SRB) (<italic>Desulfovibrio desulfuricans</italic> oral and <italic>Desulfovibrio fairfieldensis</italic> or <italic>D. desulfuricans</italic> environmental) were inoculated into the culture media (Postgate C culture medium or modified Postgate E culture medium). The biocorrosive potential of these bacteria will be an important component of a biopharmaceutical under development called BACCOR. Afterwards, four used endodontic files (UEFs) were separately inoculated into a specific culture media for 445 days at 30°C in an incubator. The four UEFs were placed in a scanning electron microscope (SEM) and analyzed by the energy-dispersive X-ray spectrometry (EDS). <italic>Results</italic>. The confocal laser scanning microscopic images indicate the presence of biofilm in the four samples. The SEM and SEM-EDS revealed the presence of rough, irregular structures adhering along the metallic surface of the used endodontic files, suggesting a mature calcified biofilm with a high concentration of Ca, P, C, and S. <italic>Conclusion</italic>. The formation of SRB biofilms on used endodontic files shows characteristics that may contribute to the biocorrosion of these files, and the results may also provide complementary data for a biopharmaceutical, which is still under development to assist in the removal of fractured endodontic files inside root channels.
- Subjects
SULFATE-reducing bacteria; CORROSIVE wastes; ENDODONTICS; DESULFOVIBRIO desulfuricans; BIOFILMS
- Publication
International Journal of Dentistry, 2018, p1
- ISSN
1687-8728
- Publication type
Article
- DOI
10.1155/2018/8303450