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- Title
INCIDENCIA DE Listeria monocytogenes EN PLANTAS DE PROCESAMIENTO DE TILAPIA SITUADAS EN EL ESTADO DE SÃO PAULO (BRASIL).
- Authors
VÁZQUEZ-SÁNCHEZ, Daniel; ANTUNES GALVÃO, Juliana; OETTERER, Marília
- Abstract
Background: A remarkable increase in the aquaculture of tilapia was observed in Brazil in the last decade due to its high commercial demand worldwide. However, this intensification in tilapia production has not been always followed by proper measures of food control, which can increase the risk of food intoxications. Objectives: The aim of this study was therefore evaluate for the first time the incidence of Listeria monocytogenes in Brazilian tilapia-processing plants. Methods: Twenty-five critical control points were investigated in two factories situated in the state of São Paulo. Raw and processed tilapias, water, and food contact surfaces including stainless steel tables, knives, workers' gloves, cutting boards and plastic crates were studied. Listeria spp. were isolated in 3M Petrifilm Environmental Listeria plates and confirmed by generous-specific prs PCR. Multiplex PCR was carried out to analyze the four major L. monocytogenes serovars (1/2a, 1/2b, 1/2c, and 4b). L. monocytogenes isolates were characterized by RAPDPCR using primers HLWL85, OPM-01 and DAF4. Cluster analysis by UPGMA based on similarity values between combined RAPD fingerprints were performed to group the isolates in different strains. Results: The presence of L. monocytogenes in the 4% and 16% of the critical control points analyzed in each factory did not comply with legal parameters in force. In particular, processed tilapias, workers' gloves, cutting boards, blood pools, stainless steel trays and plastic crates were contaminated, but at low concentrations (<1.5 log CFU/cm2). L. monocytogenes isolates were characterized in seven different strains by RAPD-PCR. Two strains carried the serotype 1/2a, four the serotype 1/2b and one the serotype 1/2c. These results allowed determine that the final product have been contaminated by L. monocytogenes in the cutting boards. Interestingly, one strain was found in both factories, although they were located 400 km away from each other, which suggest a high prevalence of this strain in tilapia-processing facilities. Conclusions: The presence of L. monocytogenes in both tilapia-processing facilities indicates the need of optimize the disinfection strategies applied, as well as revise the hygienic practices during handling and processing operations to ensure the safety of products.
- Publication
Vitae (01214004), 2016, Vol 23, pS60
- ISSN
0121-4004
- Publication type
Article