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- Title
<italic>Pelagibaca bermudensis</italic> promotes biofuel competence of <italic>Tetraselmis striata</italic> in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity.
- Authors
Patidar, Shailesh Kumar; Kim, Sae-Hee; Kim, Jin Ho; Park, Jungsoo; Park, Bum Soo; Han, Myung-Soo
- Abstract
Background: Amelioration of biofuel feedstock of microalgae using sustainable means through synthetic ecology is a promising strategy. The co-cultivation model (<italic>Tetraselmis striata</italic> and <italic>Pelagibaca bermudensis</italic>) was evaluated for the robust biofuel production under varying stressors as well as with the selected two-stage cultivation modes. In addition, the role of metabolic exudates including the quorum-sensing precursors was assessed. Results: The co-cultivation model innovated in this study supported the biomass production of <italic>T. striata</italic> in a saline/marine medium at a broad range of pH, salinity, and temperature/light conditions, as well as nutrient limitation with a growth promotion of 1.2–3.6-fold. Hence, this developed model could contribute to abiotic stress mitigation of <italic>T. striata</italic>. The quorum-sensing precursor dynamics of the growth promoting bacteria <italic>P. bermudensis</italic> exhibited unique pattern under varying stressors as revealed through targeted metabolomics (using liquid chromatography–mass spectrometry, LC–MS). <italic>P. bermudensis</italic> and its metabolic exudates mutually promoted the growth of <italic>T. striata</italic>, which elevated the lipid productivity. Interestingly, hydroxy alkyl quinolones independently showed growth inhibition of <italic>T. striata</italic> on elevated concentration. Among two-stage cultivation modes (low pH, elevated salinity, and nitrate limitation), specifically, nitrate limitation induced a 1.5 times higher lipid content (30–31%) than control in both axenic and co-cultivated conditions. Conclusion: <italic>Pelagibaca bermudensis</italic> is established as a potential growth promoting native phycospheric bacteria for robust biomass generation of <italic>T. striata</italic> in varying environment, and two-stage cultivation using nitrate limitation strategically maximized the biofuel precursors for both axenic and co-cultivation conditions (T and T-PB, respectively). Optimum metabolic exudate of <italic>P. bermudensis</italic> which act as a growth substrate to <italic>T. striata</italic> surpasses the antagonistic effect of excessive hydroxy alkyl quinolones [HHQ, 4-hydroxy-2-alkylquinolines and PQS (pseudomonas quorum signal), 2-heptyl-3-hydroxy-4(1H)-quinolone].
- Subjects
MICROALGAE; ALGAL biofuels; PLANT lipids; PLANT exudates; PLANT biomass
- Publication
Biotechnology for Biofuels, 2018, Vol 11, Issue 1, p1
- ISSN
1754-6834
- Publication type
Article
- DOI
10.1186/s13068-018-1185-x