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- Title
Detection of Legionella pneumophila in the bronchoalveolar lavage samples by real time PCR in patients with ventilator-associated pneumonia in ICU.
- Authors
Khorvash, F.; Yousefi, A.; Meidani, M.; Abassi, S.; Yaran, M.; Zolfaghari, M. R.; Rezaei, A.; Ataei, B.
- Abstract
Background and aims: Legionella pneumophila is an organism of public health interest because of its ability to cause mechanical ventilation. The aim of this research was determination of Legionella pnemophila in bronchoalveolar lavage samples in patients with ventilator-associated pneumonia by real time PCR in ICU at Al Zahra hospital. Methods: In this descriptive analytic study, Pneumonia patients hospitalized in ICU of Isfahan Al Zahra hospital were studied in one year in 2011. Thirty-nine Bronchoalveolar lavage specimens were collected from patients with ventilator associated Pneumonia and kept in- 20°C until the test. DNA was extracted by phenol chloroform and Real time PCR process was done in 45 cycles consist of 95°C for 4 seconds in the first step and 58°C for 30 seconds, while experiments were performed using Taq Man method. Results: The results for presence of Legionella pneumophila bacteria in all samples were negative. Minimum age of patients was 20 and maximum age was 86 years. Duration of hospitalization in ICU was at least 2 days and maximum was 65 days. Maximum ventilation period was 65 days and minimum period was 2 days. Conclusion: This study demonstrates the absence of Legionella pneumophila in patients with ventilator associated pneumonia in ICU of this hospital. It seems that Legionella pneumophila microbiological pattern recognition is also logical for other medical centers.
- Publication
Journal of Shahrekord University of Medical Sciences, 2015, Vol 17, Issue 1, p51
- ISSN
1735-1448
- Publication type
Article