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- Title
Identification of genes differentially expressed in SH-SY5Y neuroblastoma cells exposed to the prion peptide 106–126.
- Authors
Martínez, Tamara; Pascual, Angel
- Abstract
Prion diseases are a group of neurodegenerative disorders characterized by astrocytosis and progressive neuronal degeneration. As a causative agent, prions have been intensely investigated in different experimental models. However, the mechanisms and pathways involved in the prion-induced neurological dysfunction are poorly understood. In this work we have investigated the influence of prion infection on the gene expression profile in a human neuroblastoma cell line. Using a DNA microarray and quantitative reverse transcriptase-polymerase chain reaction methods, we have analysed in SH-SY5Y cells the effects of a synthetic peptide corresponding to the 106–126 neurotoxic region of the cellular human prion protein. Our results show that addition of this peptide to the neuronal culture specifically changes the expression of a relative high number of genes, and causes a progressive neuronal death even in the absence of microglia.
- Subjects
PRIONS; PRION disease diagnosis; NEURODEGENERATION; GENE expression; NEUROBLASTOMA; DNA microarrays; REVERSE transcriptase
- Publication
European Journal of Neuroscience, 2007, Vol 26, Issue 1, p51
- ISSN
0953-816X
- Publication type
Article
- DOI
10.1111/j.1460-9568.2007.05646.x