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- Title
Gene delivery from a DNA controlled-release stent in porcine coronary arteries.
- Authors
Klugherz, Bruce D.; Jones, Peter L.; Cui, Xiumin; Chen, Weiliam; Meneveau, Nicolas F.; DeFelice, Suzanne; Connolly, Jeanne; Wilensky, Robert L.; Levy, Robert J.
- Abstract
Expandable intra-arterial stents are widely used for treating coronary disease. We hypothesized that local gene delivery could be achieved with the controlled release of DNA from a polymer coating on an expandable stent. Our paper reports the first successful transfection in vivo using a DNA controlled-release stent. Green fluorescent protein (GFP) plasmid DNA within emulsion-coated stents was efficiently expressed in cell cultures (7.9% ± 0.7% vs. 0.6% ± 0.2% control,p < 0.001) of rat aortic smooth muscle cells. In a series of pig stent-angioplasty studies, GFP expression was observed in all coronary arteries (normal, nondiseased) in the DNA-treated group, but not in control arteries. GFP plasmid DNA in the arterial wall was confirmed by PCR, and GFP presence in the pig coronaries was confirmed by immunohisto-chemistry. Thus, DNA-eluting stents are capable of arterial transfection, and could be useful as delivery systems for candidate vectors for gene therapy of cardiovascular diseases.
- Subjects
GENE transfection; DRUG delivery devices; CORONARY arteries
- Publication
Nature Biotechnology, 2000, Vol 18, Issue 11, p1181
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/81176