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- Title
Effect of Different Dietary Protein Levels on Egg Development and its Response to Inducing Agents during Induced Spawning of Channa marulius.
- Authors
Hafeez-ur-Rehman, Muhammad; Abbas, Farzana; Ashraf, Muhammad; Narejo, Naeem Tariq; Iqbal, Khalid Javed; Abbas, Ghulam; Andleeb, Syedah
- Abstract
Brood stock of Channa marulius with an average weight of 948.02±5.72g was randomly stocked in duplicate earthen ponds (90×70×4ft) and fed on 40%, 35% and 30% protein diet @5% of their live body weight. In 40% protein diet treatment, male fish was injected 1st dose with ovaprim + HCG (0.3+0.3ml) and female fish was given 2nd dose after 24 hrs of intervals with ovaprim (0.2ml), while 1st dose ovaprim (0.7ml)+HCG (1.0ml) and 2nd dose ovaprim (0.7ml) was given to the females. The treatment which was given 35% protein diet received 1st HCG+HMG (0.3+0.3ml) and ovaprim (0.2ml) to males and ovaprim (0.5ml)+fresh PG (1.0 ml), 2nd dose ovaprim (0.7ml) and 30% protein containing feed treatment 1st dose with ovaprim+HMG (0.3+0.3ml) and 2ndovaprim (0.2ml) to males and ovaprim (0.3ml) + HMG (1.0ml) with 2nd dose ovaprim (0.7ml kg-1) to females were injected. The highest average fecundity with latency period of 47.70±0.54 h was observed in treatment 3 (40% CP) while treatment 2 (35% CP), in combination of ovaprim + fresh PG showed very short latency period when compared to the ovaprim+HCG injected fish. Treatment 1 and control failed to spawn. Fish fed on 30% protein diet (treatment 1) and the control had the lowest fecundity showing insufficiency of protein required for proper development of the ovary. These studies revealed that 40% protein diet not only improved fish growth and health but also enhanced egg fecundity, gonadal development and spawning.
- Subjects
LOW-protein diet; EGG industry; BROOD stock assessment; SPAWNING; CHANNA
- Publication
Pakistan Journal of Zoology, 2017, Vol 49, Issue 1, p337
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2017.49.1.337.343