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- Title
Study of β-Lactoglobulin Milk Protein Variants in Buffalo.
- Authors
Tahira, Iffat; Mahmood, Abid; Saqlain, Muhammad; Hanif, Nafeesa Qudsia; Raja, Ghazala Kaukab
- Abstract
Polymorphism exists in β-LG milk encoding gene in dairy animals which results in different protein isoforms being expressed m the milk. Overall quality of raw milk, total milk protein and fat content, depends on which β-LG isoforms are being expressed. Present study was planned to identify Beta-Lactoglobulin (β -LG) protein isoforms m Bubalm bubalis (Riverine buffalo) Nili Ravi breed milk and-to explore their association with major milk constituents. β-Lactoglobulin protein variants were typed using sodium dodecyl sulfate Polyacrylamide gel electrophoresis (SDS-PAGE). Total milk protein and whey contents were estimated using Bradford 1976 method while total milk fat by Gerber method. Three β-LG protein isoforms AA, BB and AB were identified with genotype frequencies; 0.49, 0.10 and 0.40 respectively. Allele A, with higher frequency of 0.69, was found more prevalent in studied animals as compared to B allele. The AA and AB isoforms were associated with high total milk protein contents (4.36%) while AB isoform had high whey protein (1.0%) and total milk fat (6.06%) contents Overall we report three β-LG protein isoforms (AA, AB and BB) in Nili Ravi buffalo milk which show significant association with major milk constituents.
- Subjects
LACTOGLOBULINS; MILK proteins; RAW milk; WATER buffalo milk yield; DAIRY cattle
- Publication
Pakistan Journal of Zoology, 2014, Vol 46, Issue 2, p549
- ISSN
0030-9923
- Publication type
Article