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- Title
Crystallographic Studies on Damaged DNAs: III. N4-Methoxycytosine Can Form Both Watson-Crick Type and Wobbled Base Pairs in a B-Form Duplex1.
- Authors
Hossain, M. Tofazzal; Chatake, Toshiyuki; Hikima, Takaaki; Tsunoda, Masaru; Sunami, Tomoko; Ueno, Yoshihito; Matsuda, Akira; Takénaka, Akio
- Abstract
To investigate the mutation mechanism of purine transition in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo4CGCG), where mo4C is 2′-deoxy-N4-methoxycytidine, has been synthesized and its crystal structure determined. Two dodecamers form a B-form duplex. Electron density maps clearly show that one of the two mo4C residues forms a pair with a guanine residue of the opposite strand, the geometry being the canonical Watson-Crick type, and that the other mo4C residue forms a wobble pair with the opposite guanine residue. These two pairings are ascribed to the tautomerization of the methoxylated cytosine moieties between the amino and imino forms.
- Subjects
CYTOSINE; DNA; GUANINE; ELECTRON density; GENETIC mutation
- Publication
Journal of Biochemistry, 2001, Vol 130, Issue 1, p9
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a002967