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- Title
Efficacy of specific IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model.
- Authors
Zhen, Y.-H.; Fang, R.; Ding, C.; Jin, L.-J.; Li, X.-Y.; Diao, Y.-P.; Shu, X.-H.; Ma, X.-C.; Xu, Y.-P.
- Abstract
Aims: To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model. Methods and Results: Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity to LPS when subjected to an ELISA. Endotoxemia was induced in mice by intraperitoneal injection of LPS at a dose of 20 mg kg−1 for measuring survival rate and 10 mg kg−1 for cytokine measurement. The survival rate of mice treated with 200 mg kg−1 specific IgY or 5 mg kg−1 dexamethasone was 70% while none of the mice in the normal saline-treated group survived more than 7 days. Specific IgY significantly ( P < 0·05) decreased tumour necrosis factor-α (TNF-α) level and increased interleukin-10 (IL-10) level in the serum of endotoxemia mice. Specific IgY had less of an effect on TNF-α than dexamthasone, while its effect on increasing IL-10 was stronger than dexamethasone. Haematoxylin and eosin-stained sections indicated that IgY attenuated the damage to the lung and liver observed in mice with endotoxemia. Conclusions: The specific IgY increased the survival rate of mice with endotoxemia induced by LPS, down-regulated TNF-α and up-regulated IL-10 in serum and attenuated the extent of damage to the lung and liver. Significance and Impact of the Study: The specific IgY has potential for the treatment of LPS-induced endotoxemia.
- Subjects
IMMUNOGLOBULINS; EGG yolk; ENDOTOXINS; ENDOTOXEMIA; LABORATORY mice
- Publication
Journal of Applied Microbiology, 2011, Vol 111, Issue 6, p1524
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/j.1365-2672.2011.05155.x