We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Co-induction of methyltransferase Rv0560c by naphthoquinones and fibric acids suggests attenuation of isoprenoid quinone action in Mycobacterium tuberculosis.
- Authors
Garbe, Thomas R.
- Abstract
The superoxide generator menadione was previously demonstrated as an inducer of growth stage dependent protein patterns in Mycobacterium tuberculosis. The present study refines this observation by characterizing a novel 27-kDa protein that had not been observed in previous studies relying on younger cultures, A very similar response, based on two-dimensional gel electrophoretic analyses, was induced by the closely related naphthoquinone plumbagin. The 27-kDa protein was also induced by the pro-oxidant peroxisome proliferator gemfibrozil and to a lesser extent by the structurally related compounds fenofibrate and clofibrate. N-terminal sequence data of proteolytic fragments from the 27-kDa protein demonstrated its identity with protein Rv0560c. previously demonstrated to be inducible by salicylate, which also possesses peroxisome proliferating properties. Protein Rv0560c bears three conserved motifs characteristic of S- adenosylmethionine-dependent methyltransferases, Further sequence similarities suggest a function in the biosynthesis of isoprenoid compounds, e.g., tocopherol, ubiquinone, and sterois. Such involvement is supported by the recognized yet unexplained widespread interference of menadione, salicylate, and fibrates with the isoprenoid quinones ubiquinone, menaquinone, and vitamin K. Induction of Rv0560c by fibrates, salicylate, and naphtboquinones is thus suggested to be caused by action on the plasma membrane, reminiscent of cytochrome P450BM-3 induction by fibrates in Bacillus megaterium, which catalyzes the hydroxylation of fatty acids and thus modulates membrane properties.
- Subjects
MYCOBACTERIUM tuberculosis; METHYLTRANSFERASES; ISOPENTENOIDS; BIOSYNTHESIS; VITAMIN E; VITAMIN K
- Publication
Canadian Journal of Microbiology, 2004, Vol 50, Issue 10, p771
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W04-067