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- Title
A Total Flavonoids Extract of Scurrula parasitic L. Parasitized on Nernium indicum Mill. Inducing Human Burkitt's Lymphoma Cell Line CA46 Apoptosis and the Related Molecular Mechanism.
- Authors
XIAO Yi-jun; LIU Fen; CHEN Yuan-zhong; CHEN Jian-hua; CHEN Bing-hua; FAN Yan-li
- Abstract
The anticancer activity of a total flavonoids extract of Scurrula parasitic L. parasitized on Nernium indicum Mill. (Nispex) on human Burkitt's lymphoma cell line CA46 and its molecular mechanism was studied here. The growth inhibition activity of CA46 cell was detected by MTF assay and colony formation assay. The cell apoptosis induced by Nispex was detected by AO/EB fluorescence staining, TUNEL analysis, DNA fragmentation assay and Annexin V-FITC/PI flow cytometry assay, and the expression of protein related with cell apoptosis was detected by Western blot assay. The results showed that Nispex remarkably inhibited the proliferation of CA46 cell in a dose-dependent and time-dependent mariner, with IC50 value of 1.72 µg/mL. Concomitantly, Nispex induced CA46 celt apoptosis also. The expressions of Bax, Caspase-3 protein were enhanced and NF-κBp65, Bcl-2, PARP protein were decreased after Nispex treatment in CA46 cell. These results indicated that Nispex could efficiently inhibit cancer cell' s growth and induce its apoptosis; the inhibition of NF-κB signal pathway may be involved in the process of CA46 cell apoptosis induced by Nispex.
- Subjects
FLAVONOIDS; PLANT extracts; ANTINEOPLASTIC agents; LYMPHOMAS; APOPTOSIS
- Publication
Natural Product Research & Development, 2008, Vol 20, Issue 5, p797
- ISSN
1001-6880
- Publication type
Article