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- Title
Plasma levels of interleukin 27 in falciparum malaria is increased independently of co-infection with HIV: potential immune-regulatory role during malaria.
- Authors
Otterdal, Kari; Berg, Aase; Michelsen, Annika E.; Patel, Sam; Gregersen, Ida; Sagen, Ellen Lund; Halvorsen, Bente; Yndestad, Arne; Ueland, Thor; Langeland, Nina; Aukrust, Pål
- Abstract
<bold>Background: </bold>The immune response during falciparum malaria mediates both harmful and protective effects on the host; however the participating molecules have not been fully defined. Interleukin (IL)-27 is a pleiotropic cytokine exerting both inflammatory and anti-inflammatory effects, but data on IL-27 in malaria patients are scarce.<bold>Methods: </bold>Clinical data and blood samples were collected from adults in Mozambique with P. falciparum infection, with (n = 70) and without (n = 61) HIV-1 co-infection, from HIV-infected patients with similar symptoms without malaria (n = 58) and from healthy controls (n = 52). In vitro studies were performed in endothelial cells and PBMC using hemozoin crystals. Samples were analyzed using enzyme immunoassays and quantitative PCR.<bold>Results: </bold>(i) IL-27 was markedly up-regulated in malaria patients compared with controls and HIV-infected patients without malaria, showing no relation to HIV co-infection. (ii) IL-27 was correlated with P. falciparum parasitemia and von Willebrand factor as a marker of endothelial activation, but not with disease severity. (iii) In vitro, IL-27 modulated the hemozoin-mediated cytokine response in endothelial cells and PBMC with enhancing effects on IL-6 and attenuating effects on IL-8.<bold>Conclusion: </bold>Our findings show that IL-27 is regulated during falciparum malaria, mediating both inflammatory and anti-inflammatory effects, potentially playing an immune-regulatory role during falciparum malaria.
- Subjects
MOZAMBIQUE; MALARIA; MIXED infections; VON Willebrand factor; VON Willebrand disease; ENZYME-linked immunosorbent assay; ENDOTHELIAL cells; INTERLEUKINS; PROTOZOA; PARASITEMIA; MONONUCLEAR leukocytes; CELL culture; CROSS-sectional method; RESEARCH funding; AIDS-related opportunistic infections; EPITHELIAL cells; HIV; LONGITUDINAL method; HEMOPROTEINS
- Publication
BMC Infectious Diseases, 2020, Vol 20, Issue 1, p1
- ISSN
1471-2334
- Publication type
journal article
- DOI
10.1186/s12879-020-4783-8