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- Title
Genetic heterogeneity of L-Zagreb mumps virus vaccine strain.
- Authors
Kosutic-Gulija, Tanja; Forcic, Dubravko; Šantak, Maja; Ramljak, Ana; Mateljak-Lukacevic, Sanja; Mazuran, Renata
- Abstract
Background: The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous. Results: We identified the heterogeneity of L-Zagreb throughout the entire genome. Two major variants were defined: variant A being identical to the consensus sequence of viral seeds and vaccine(s) and variant B which differs from variant A in three nucleotide positions. The difference between viral variants in L-Zagreb strain is insufficient for distinct viral strains to be defined. We demonstrated that proportion of variants in L-Zagreb viral population depends on cell substrate used for viral replication in vitro and in vivo. Conclusion: L-Zagreb strain should be considered as a single strain composed of at least two variant viral genomes.
- Subjects
MUMPS vaccines; VIRAL vaccines; VIRAL genomes; NUCLEOTIDES; VIRAL replication
- Publication
Virology Journal, 2008, Vol 5, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/1743-422X-5-79