We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Application of a benchtop colorimetric method for quantification of blood propofol levels.
- Authors
Voss, Logan J.; Voss, John C.; Sleigh, Jamie W.
- Abstract
Quantification of plasma propofol (2,6-diisopropylphenol) in the context of clinical anaesthesia is challenging because of the need for offline blood sample processing using specialised laboratory equipment and techniques. In this study we sought to refine a simple procedure using solid phase extraction and colorimetric analysis into a benchtop protocol for accurate blood propofol measurement. The colorimetric method based on the reaction of phenols (e.g. propofol) with Gibbs reagent was first tested in 10% methanol samples (n = 50) containing 0.5-6.0 µg/mL propofol. Subsequently, whole blood samples (n = 15) were spiked to known propofol concentrations and processed using reverse phase solid phase extraction (SPE) and colorimetric analysis. The standard deviation of the difference between known and measured propofol concentrations in the methanol samples was 0.11 µg/mL, with limits of agreement of - 0.21 to 0.22 µg/mL. For the blood-processed samples, the standard deviation of the difference between known and measured propofol concentrations was 0.09 µg/mL, with limits of agreement - 0.18 to 0.17 µg/mL. Quantification of plasma propofol with an error of less than 0.2 µg/mL is achievable with a simple and inexpensive benchtop method.
- Subjects
PROPOFOL; SOLID phase extraction; COLORIMETRIC analysis; STANDARD deviations; HIGH performance liquid chromatography; RESEARCH evaluation; COLORIMETRY
- Publication
Journal of Clinical Monitoring & Computing, 2021, Vol 35, Issue 6, p1485
- ISSN
1387-1307
- Publication type
journal article
- DOI
10.1007/s10877-020-00616-6