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- Title
In vitro efficacy of the essential oil of Piper cubeba L. (Piperaceae) against Schistosoma mansoni.
- Authors
Magalhães, Lizandra; Souza, Julia; Wakabayashi, Kamila; Laurentiz, Rosangela; Vinhólis, Adriana; Rezende, Karen; Simaro, Guilherme; Bastos, Jairo; Rodrigues, Vanderlei; Esperandim, Viviane; Ferreira, Daniele; Crotti, Antônio; Cunha, Wilson; Silva, Márcio
- Abstract
In this paper, cercariae, schistosomula, and adult Schistosoma mansoni worms were incubated in vitro with the essential oil of Piper cubeba (PC-EO) at concentrations from 12.5 to 200 μg/mL, and the viability was evaluated using an inverted microscopy. The effects of PC-EO at 100 and 200 μg/mL on the stages of S. mansoni were similar to those of the positive control (PZQ at 12.5 μg/mL), with total absence of mobility after 120 h. However, at concentrations from 12.5 to 50 μg/mL, PC-EO caused a reduction in the viability of cercariae and schistosomula when compared with the negative control groups (RPMI 1640 or dechlorinated water) or (RPMI 1640 + 0.1% DMSO or dechlorinated water + 0.1% DMSO). On the other hand, adult S. mansoni worms remained normally active when incubated with PC-EO at concentrations of 12.5 and 25 μg/mL, and their viabilities were similar to those of the negative control groups. In addition, at concentrations ranging from 50 to 200 μg/mL, separation of all the coupled adult worms was observed after 24 h of incubation, which is related to the fact of the reduction in egg production at this concentration. The main chemical constituents of PC-EO were identified by gas chromatography-mass spectrometry as being sabinene (19.99%), eucalyptol (11.87%), 4-terpineol (6.36%), β-pinene (5.81%), camphor (5.61%), and δ-3-carene (5.34%). The cytotoxicity of the PC-EO was determined, and a significant cytotoxicity was only obtained in the concentration of 200 μg/mL after 24 h treatment. The results suggest that PC-EO possesses an effect against cercariae, schistosomula, and adult worms of the S. mansoni.
- Subjects
ESSENTIAL oils; PIPERACEAE; SCHISTOSOMA mansoni; WORM eggs; EGG incubation; CONTROL groups; GAS chromatography/Mass spectrometry (GC-MS)
- Publication
Parasitology Research, 2012, Vol 110, Issue 5, p1747
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-011-2695-7