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- Title
In-vitro and in-vivo evaluation of austocystin D liposomes.
- Authors
Li, Shuo; Hu, Jie; Zhang, Linan; Zhang, Li; Sun, Yongjun; Xie, Yinghua; Wu, Shaomei; Liu, Lei; Gao, Zibin
- Abstract
Objectives The purpose this study is to enhance the anti-tumour activity of austocystin D ( AD) by AD-loaded liposomes ( AD- Ls). Methods AD- Ls were prepared by the film dispersion-ultrasonication method and characterized in terms of particle size and zeta potential, encapsulation efficiency and in-vitro drug release. In vivo, the pharmacokinetics, biodistribution and anti-tumour effect were also compared with those of the solution. Key findings The obtained liposomes were a mildly translucent suspension, with a particle size of 71.26 ± 6.43 nm, a polydispersity index of 0.259 ± 0.017 and a zeta potential of −9.9 ± 1.8 m V. Transmission electron microscope examination showed that the liposomes had a spherical shape and a multilayer structure. The encapsulation efficiency of AD- Ls was 83.74 ± 1.26%. AD was continuously released from liposomes up to 72 h in in-vitro experiments. The growth of HT-29 tumours in animal models was controlled more effectively by AD-LS than by AD solution. Pharmacokinetic study showed that AD- Ls had higher t½β and mean retention time. Biodistribution results in tumour-bearing mice showed that the AD-LS could target to liver and tumour. Conclusions This study indicates that AD- Ls are a potential carrier of AD for the treatment of tumours in the liver, increasing the cure efficiency and decreasing the side effects on other tissues.
- Subjects
LIPOSOMES; PHARMACOKINETICS; CELL lines; COLON tumors; LABORATORY mice; CANCER cells
- Publication
Journal of Pharmacy & Pharmacology, 2013, Vol 65, Issue 3, p355
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/j.2042-7158.2012.01606.x