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- Title
Vaccine and oncogenic strains of gallid herpesvirus 2 contain specific subtype variations in the 5′ region of the latency-associated transcript that evolve in vitro and in vivo.
- Authors
Labaille, Jennifer; Lion, Adrien; Boissel, Elodie; Trapp, Sascha; Nair, Venugopal; Rasschaert, Denis; Dambrine, Ginette
- Abstract
Gallid herpesvirus 2 (GaHV-2) is the alphaherpesvirus responsible for Marek's disease (MD), a T-cell lymphoma of chickens. The virulence of the GaHV-2 field strain is steadily increasing, but MD is still controlled by the CVI988/Rispens vaccine. We tried to determine distinguishing traits of the CVI988/Rispens vaccine by focusing on the 5′ end region of the latency-associated transcript (5′ LAT). It includes a variable number of 60-bp tandem repeats depending on the GaHV-2 strain. By analyzing six batches of vaccine, we showed that CVI988/Rispens consisted of a population of 5′ LAT molecular subtypes, all with deletions and lacking 60-bp tandem repeat motifs, with two major subtypes that probably constitute CVI988/Rispens markers. Serial passages in cell culture led to a substantial change in the frequency of CVI988/Rispens 5′ LAT subtypes, with non-deleted subtypes harboring up to four 60-bp repeats emerging during the last few passages. Dynamic changes in the distribution of 5′ LAT-deleted subtypes were also detected after infection of chickens. By contrast, the 5′ LAT region of the oncogenic clonal RB-1B strain, which was investigated at every step from the isolation of the clonal bacmid RB-1B DNA to the isolation of the ovarian lymphoma cell line, consisted of non-deleted 5′ LAT subtypes harboring at least two 60-bp repeats. Thus, vaccine and oncogenic GaHV-2 strains consist of specific populations of viral genomes that are constantly evolving in vivo and in vitro and providing potential markers for epidemiological surveys.
- Subjects
MAREK'S disease virus; HERPESVIRUS vaccines; MICROBIAL virulence; ONCOGENES; T-cell lymphoma; GENETIC transcription; IN vitro studies
- Publication
Archives of Virology, 2015, Vol 160, Issue 1, p161
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-014-2248-3