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- Title
Endometrial mRNA expression of matrix metalloproteinases, their tissue inhibitors and cell adhesion molecules in unexplained infertility and implantation failure patients.
- Authors
Konac, Ece; Alp, Ebru; Onen, Hacer Ilke; Korucuoglu, Umit; Biri, Aydan Asyali; Menevse, Sevda
- Abstract
The aim of this study was to analyse whether some cases of unexplained infertility and implantation failure after IVF could be explained by different expression levels of the matrix metalloproteinases (MMP-2, 9), their tissue inhibitors (TIMP-2, 3) and intercellular (ICAM-1) and vascular (VCAM-1) adhesion molecules in endothelial cells. Total RNA was extracted from the endometrial tissues of 41 women (unexplained infertile, group 1, n = 15; fertile volunteers, group 2, n = 15 and patients with implantation failure after IVF, group 3, n = 11). MMP-2, MMP-9, TIMP-2, TIMP-3, ICAM-1 and VCAM-1 mRNA expression levels were measured quantitatively using real-time polymerase chain reaction. In the endometrium from women with unexplained infertility and implantation failure after IVF, MMP-2 and TIMP-3 expression were significantly decreased when compared with the fertile group (P < 0.05 and P ≤ 0.001 respectively). In addition, a marked decrease was observed in the expression of VCAM-1 in women with unexplained infertility. These results suggest that the expression of gelatinase A (MMP-2), TIMP-3 and VCAM-1, at least at the transcriptional level, might be regulated by common factors and signalling pathways. The present study adds new and important data in this field and highlights the complex preparation of the endometrium for implantation at the molecular level.
- Subjects
MESSENGER RNA; ENDOMETRIUM; METALLOPROTEINASES; CELL adhesion molecules; INFERTILITY; HUMAN embryo transfer; HUMAN in vitro fertilization
- Publication
Reproductive BioMedicine Online (Reproductive Healthcare Limited), 2009, Vol 19, Issue 3, p391
- ISSN
1472-6483
- Publication type
Article
- DOI
10.1016/S1472-6483(10)60174-5