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- Title
Whole exome sequencing identifies a novel SCN1A mutation in genetic (idiopathic) generalized epilepsy and juvenile myoclonic epilepsy subtypes.
- Authors
Chan, Chung-Kin; Low, Joyce Siew-Yong; Lim, Kheng-Seang; Low, Siew-Kee; Tan, Chong-Tin; Ng, Ching-Ching
- Abstract
<bold>Introduction: </bold>Genetic (idiopathic) generalized epilepsy (GGE) is a common form of epilepsy characterized by unknown aetiology and a presence of genetic component in its predisposition.<bold>Methods: </bold>To understand the genetic factor in a family with GGE, we performed whole exome sequencing (WES) on a trio of a juvenile myoclonic epilepsy/febrile seizure (JME/FS) proband with JME/FS mother and healthy father. Sanger sequencing was carried out for validation of WES results and variant detection in other family members.<bold>Results: </bold>Predictably damaging variant found in affected proband and mother but absent in healthy father in SCN1A gene was found to be associated with generalized epilepsy and febrile seizure. The novel non-synonymous substitution (c.5753C>T, p.S1918F) in SCN1A was found in all family members with GGE, of which 4/8 were JME subtypes, and/or febrile seizure, while 3 healthy family member controls did not have the mutation. This mutation was also absent in 41 GGE patients and 414 healthy Malaysian Chinese controls.<bold>Conclusion: </bold>The mutation is likely to affect interaction between the sodium channel and calmodulin and subsequently interrupt calmodulin-dependent modulation of the channel.
- Subjects
CALMODULIN; EPILEPSY; FEBRILE seizures; SODIUM channels; ETIOLOGY of diseases
- Publication
Neurological Sciences, 2020, Vol 41, Issue 3, p591
- ISSN
1590-1874
- Publication type
journal article
- DOI
10.1007/s10072-019-04122-9