We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of expression of genes involved in proliferation, apoptosis and cell adhesion in colorectal cancer.
- Authors
Murăraşu, Daniela; Mihalcea, Corina-Elena; Cinca, Sabin; Marincaş, Augustin; Cirimbei, Ciprian; Simion, Laurenţiu; Puiu, Liliana
- Abstract
In the colorectal tumorigenesis process, there are multiple molecular events which change the expression profiles of certain genes involved in essential cell processes for the normal operation of the cell: cellular cycle and proliferation, apoptosis and cell adhesion. Protein p53 is the most important tumour suppressor and one of the most investigated molecular prognosis and prediction markers in the colorectal cancer (CRC); it transcriptionally regulates numerous genes or indirectly influences the activity of certain proteins. The purpose of the paper was the analysis of the expression of a set of 10 genes (TP53, TP53INP1, WRAP53, KLF4, EZH2, PLK1, BIRC5, SPARC, CDH1 and TACSTD1), involved in proliferation, apoptosis and cellular adhesion and which are linked to the activity of p53, the identification of molecular parameters useful in staging and prognosis of malignant diseases, as well as the correlations between the expression of these genes and the mRNA levels of TP53 in CRC. Materials and methods. Fourty fragments of tumour tissue and apparently normal adjacent mucous membranes collected from patients with CRC subject to surgery, within the “Prof. Dr. Alex. Trestioreanu” Institute of Oncology Bucharest have been analysed. From the samples, total RNA was isolated using Trizol (Thermofisher Scientific, USA), which was purified with the kit RNeasy Mini Kit 50 (Qiagen, Thermofisher Scientific) and reverse-transcribed in cDNA. The levels of tRNA of the 10 genes were measured through the semiquantitative detection of fluorescence in real time with the help of the device 7900 HT (Thermofisher Scientific) and of the pre-optimised probes TaqMan. The data obtained have been analysed with the programs SDS and RQ Manager 1.2 through the method ΔΔCt. The studies of correlation between the analysed parameters were performed with the program GraphPad Prism 5.0 (GraphPad Software Inc). Outcomes. In the CRC tumours, significant variations were noticed in the expression of the genes BIRC5, EZH2, PLK1, TP53, TACSTD1, CDH1, KLF4 and TP53INP1, when compared to normal tissue. The expression of the gene TP53 was correlated to the majority of the examined genes, except for CDH1, TACSTD1 and SPARC (in the normal tissues) and KLF4 and SPARC (in the tumour tissue). The increase in the expression levels of PLK1 and KLF4 was noticed in old patients and respectively in men. At the level of the tumours in the proximal colon, the decrease in the expression of CDH1 and TACSTD1 was noticed. Neoplasms with serous infiltration have been characterised by an increase in the expression of the genes CDH1, KLF4, TACSTD1 and TP53INP1 and a low expression of WRAP53, while the tumours at the level of the nodes have had a significant increase in the expression of the genes BIRC5, EZH2 and PLK1. At the same time, with the progression of the disease, the increase in the expression of the genes EZH2, TP53 and WRAP53 and the decrease in the expression of CDH1, KLF4 and TP53INP1 were noticed. In tumours with distal metastases, the expression of the genes KLF4, SPARC and TP53INP1 decreased significantly, while the expression of TP53 increased. Conclusions. Except for SPARC and WRAP53, the expression of the other 8 genes analysed represents biomarkers for the CRC tumours confirmed in this study. The expression of the p53 tumour suppressor is highly correlated with the genes involved in cell proliferation. The levels of expression of the genes TP53, TP53INP1, KLF4 and CDH1 represent parameters which may be useful in the prognosis of the development of distal metastases.
- Subjects
GENE expression; COLON cancer; APOPTOSIS
- Publication
Oncolog-Hematolog, 2017, Issue 39, p54
- ISSN
2066-8716
- Publication type
Abstract