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- Title
两株茶叶籽水浆发酵杆菌的分离鉴定 及其数量动态分析.
- Authors
任翠娟; 姜金仲; 王自布; 穆安德; 王兴春; 黄燕芬
- Abstract
Abstract:【Objective】The species and count dynamic of microorganism in fermentation layering of Camellia sinensis water milk were clarified to provide reference for optimization of fermentation technique of C. sinensis and revealing the fermentation layering mechanism of C. sinensis water milk.【Method】C. sinensis water milk that fermented for 5 h was cultured on MRS solid culture medium with cycloheximide. The microorganism was isolated and purified. Through morphological observation,enzymatic reaction,carbon source reaction gene sequencing and comparison of 16S rDNA and pheS,establishment and analysis of phylogenetic tree,the isolated microorganism were identified. Spread plate method was used to observe the dynamic amount of bacteria.【Result】Two strains were isolated and purified from fermented C. sinensis water milk,their serial numbers were JJZ12 and JJZ21 respectively. The colony of JJZ12 was round,white and convex,with smooth and moist surface and neat edges;and its thallus were rod-shaped,with a size of 0.6-1.2 μm×1.0- 3.0 μm,and in pairs or short chains,gram-positive. The morphology of JJZ21 colony was the same as JJZ12. However, its size of thallus was 0.5-0.7 μm×0.8-1.7 μm,and single or paired,gram positive. JJZ12 and JJZ21 were authenticated as a strain of Gluconacetobacter liquefaciens and Lactobacillus plantarum subsp. plantarum respectively. Combined with the research number,the full name of these two zymobacter strains were G. liquefaciens JJZ12 and L. plantarum subsp. plantarum JJZ21. In the fermentation process of C. sinensis water milk,the total number of JJZ12 strains and JJZ21 strains in fermented milk increased rapidly after 5 h,and peaked at 15 h,and after that gradually reduced to a stable status.【Conclusion 】JJZ12 and JJZ21 are two strains that play a major role in the middle and later periods of C. sinensis water milk fermentation as the fermentation microorganism. The conclusion afford preliminary evidences for explaining occurrence mechanism of fermentation layering of C. sinensis water milk.
- Publication
Journal of Southern Agriculture, 2018, Vol 49, Issue 8, p1603
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j.issn.2095-1191.2018.08.20