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- Title
A Group of Hypermethylated miRNA Genes in Breast Cancer and Their Diagnostic Potential.
- Authors
Filippova, E. A.; Loginov, V. I.; Pronina, I. V.; Khodyrev, D. S.; Burdennyy, A. M.; Kazubskaya, T. P.; Braga, E. A.
- Abstract
miRNA genes play an important role in cancer pathogenesis, while they may be suppressed by hypermethylation. Here, we assess the diagnostic potential of a group of hypermethylated miRNA genes (MIR-124-1, MIR-124-3, MIR-125B-1, MIR-127, MIR-132, MIR-193a, and MIR-34b/c) in a representative set of 70 breast cancer samples and 17 breast tissue samples from deceased donors with no malignancies. For these seven genes, the methylation status is determined using the methylation-specific PCR. Methylation reached 26–76% in tumor specimens, 1‒27% in paired considered normal breast tissues, and 0–18% in breast tissue from deceased donors. By quantitative RT-PCR, reduced expression levels of the investigated miRNAs are detected, with a negative correlation of expression levels with gene hypermethylation. Combinations of three or four hypermethylation biomarkers, namely, MIR-124-1, MIR-125B-1, MIR-127, and MIR-34b/c are found suitable for breast cancer diagnostics; with sensitivity (76‒93%), specificity (88‒100%), and AUC (0.88‒0.94). Notably, the MIR-127 gene was hypermethylated only in the tumor samples of patients with metastases, and, therefore, should be tested as a marker of breast cancer dissemination. These findings may lead to improvement in the management of breast cancer.
- Subjects
BRCA genes; P16 gene; MICRORNA; METASTASIS; CARCINOGENESIS; BREAST cancer
- Publication
Molecular Biology, 2019, Vol 53, Issue 3, p371
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1134/S0026893319030051