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- Title
Toprak Kökenli Miksobakteri İzolatlarından Alfa-Amilaz Enzimi Üretimi ve Saflaştırılması.
- Authors
Ekinci, Melike Baran; Karahan Çakmakçı, Aynur Gül
- Abstract
In this study, 19 myxobacteria strains were isolated from 28 soil samples in the different habitats. They were collected from different provinces (Antalya, Konya and Isparta) and their districts (Kulu, Şarkikaraağaç and Atabey). Among the 19 myxobacteria isolated, Myxococcus sp KK2 was determined to have the highest α-amylase activity. With protein precipitation, enzyme purity was increased by using 40, 65 and 80% ammonium sulfate concentrations in the first application, then 20, 40 and 65% concentrations in the second application. By using dialysis and gel filtration methods, the activity of the enzyme obtained in the first application reached 883.27 U while enzyme purity increased 28.35 times, and enzyme activity was 1754.99 U after the second application while enzyme purity increased 810.24 times.
- Subjects
AMMONIUM sulfate; SOIL sampling; MYXOBACTERALES; ENZYMES; DIALYSIS (Chemistry)
- Publication
Academic Food Journal / Akademik GIDA, 2022, Vol 20, Issue 4, p398
- ISSN
1304-7582
- Publication type
Article
- DOI
10.24323/akademik-gida.1224357