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- Title
Aspergillus sojae Tarafından Üretilen Poligalakturonazın Kısmi Saflaştırılması için Kromatografik Bir Yaklaşım.
- Authors
Sen, Ilknur; Mata-Gomez, Marco A.; Rito-Palomares, Marco; Tari, Canan; Dinç, Melike
- Abstract
The aim of this study was to produce polygalacturonase from A. sojae mutant and partially purify the crude extract by chromatographic methods. As a preliminary step for the confirmation of its peptides, matrix-assisted laser-desorptionionization-time-of-flight mass spectrometry (Maldi-TOF MS) analysis was performed on in-gel digested sodiumdodecyl-sulphate-polyacrylamide-gel-electrophoresis (SDS-PAGE) gels. Three different carbon sources were employed in submerged and solid-state fermentations for the production of polygalacturonase. Crude extract was first purified by ion-exchange chromatography (IEXC) and followed further by size exclusion chromatography. Crude extracts obtained from sub-merged [of bitter orange peel, sugar beet molasses and (NH4)2SO4] and solid-state [of wheat bran, sugar beet and HCl] fermentation exhibited high levels of polygalacturonase enzyme activity (95.22 and 50.27 U/mL, respectively). Size exclusion of IEXC pooled fraction (180, 200 and 220 mM salt fractions) revealed the highest yield (36%) and purification fold (2.00). The likely polygalacturonase bands from SDS-PAGE were in-gel digested and analyzed by Maldi-TOF MS in route for peptides confirmation.
- Subjects
POLYGALACTURONASE; GEL permeation chromatography; WATER purification
- Publication
Academic Food Journal / Akademik GIDA, 2017, Vol 15, Issue 1, p8
- ISSN
1304-7582
- Publication type
Article
- DOI
10.24323/akademik-gida.305767